Previous studies on human dermatophytosis in the west bank of jordan have revealed that the animal source represented about 40o of all etio logical agents identified 2, 22. The keratinolytic nature of these fungi makes it possible to isolate them from soil by implanting human hairs, nails, pig hairs, goat hairs, buffalo hairs, sheep hairs, cow. Incidence of keratinophilic fungi from the selected soils of. Research article isolation and molecular identification of. Keratinophilic fungi from soil of brittany, france springerlink. In extreme cases, the hairs were reduced to hollow tubes resembling soda straws. In some hairs, fungal hyphae had penetrated to the center of the hair and consumed the central medullary cells. Among other nondermatophytic filamentous keratinophilic fungi known to cause human infections are species of fusarium, scopulariopsis, aspergillus. Isolation of the fungi was performed by hair bait technique. The soil keratinophilic fungi are responsible for the breakdown of any keratin containing wastes such as hair, fur and feather. Out of the 40 soil samples examined from different habitats, 30 75% were positive for the presence of keratinophilic fungi, yielding 36 isolates of keratinophilic fungi. Isolation and identification of microbial and fungal flora. The most frequently found species werefusarium moniliforme, penicillium viridicatum and an unidentified species ofacremonium.
Keratinophilic fungi on cats and dogs medical mycology. The majority consists of microscopic filaments called hyphae, and the network of filaments is the mycelium. Nondermatophytic filamentous keratinophilic fungi an citeseerx. Keratinophilic fungi from feathers of indian poultry birds. Keratinophilic fungi distribution, pathogenicity and.
The effect of ph, temperature, carbon and nitrogen sources on fungal growth and sporulation was evaluated. However, the existence of their relatives is of ancient origin. Extreme degradation of human hair by keratinophilic and. Padhye and a holiday visit to the galapagos islands provided an opportunity to collect soil specimens and study them for the presence of keratinophilic fungi. Our knowledge of ecology and epidemiology of dermatophytes and the factors influencing their transmission has helped us understand. Introduction soil is potential sources of fungal infection agent for humans and animals and well known to support the transient of keratinophilic fungi 1,2. As mentioned before, keratin is found in the outer layer of the skin, the epidermis, nails and hair and this is what the fungi feeds on. Isolation of keratinophilic fungi from soils the keratinophilic fungi were isolated from different soil samples using hair baiting technique vanbreuseghem 1952.
Rajak et al 1991, sharma et al 2008 etc are carried further this work. So if you have an outbreak of ringworm, you basically have fungi growing in the epidermis. The human hair harbors several species of fungi and also bacteria. Characterization of keratinophilic fungi in soil of semi arid region of india on free shipping on qualified orders. Isolation and identification of keratinophilic fungi from. The aim of this study was to isolate and identify keratinophilic fungi from the soil of different parks in shiraz. Employ hair baits as described by vanbreuseghem 1952 using defatted hair. Several investigations have been done in various part of iran during the recent years and showed that a rich variety of keratinophilic fungal flora exists in the soils of studied area, 14, 22, 23. The user has requested enhancement of the downloaded file. Keratinophilic fungi on sheep hairs from the west bank of. Matsushimamyces, a new genus of keratinophilic fungi from soil in. Data on the distribution of keratinophilic fungi in the soils of kaziranga national park assam are given in table 1.
It is easily detected due to its characteristic lightbulb shape. For this purpose, sterile petri dishes were half filled with the soil samples and moistened with. Microsporum gypseum complex represented by 16 isolates of m. A total of 196 soil samples from 43 parks were collected. International journal of environment agriculture and biotechnologyissn.
Classification of fungi morphology and structure pathogenicity diagnosis useful properties of fungi diverse group of chemo heterotrophs over 100,000 fungal species identified only about 100 are human or animal pathogens saprophytes digest dead organic matter. The growth of 9 species of keratinophilic fungi belonging to the genera keratinomyces, microsporum, trichophyton and epidermophyton was studied in vitro on hair, hedgehog spine, nail and callus. Study of keratinophilic fungi in madhya pradesh in madhya pradesh study on keratinophilic fungi started in 1976 by kushwaha and agrawal. Pdf keratinophilic fungi are an ecologically important group of fungi that cycle one of the most abundant and highly stable animal proteins on. Isolation of keratinophilic fungi from soil dates back to the middle of the century when a hair baiting technique was described. Diversity of keratinophilic fungi on human hairs and nails at. The frequent occurrence of chrysosporium as a geophilic keratinophilic fungus in jamaican soil is in agreement with that recorded in surveys of keratinophilic fungi of soils in several other countries 4,5,10. Other species of keratinophilic fungi isolated were gymnascella dankaliensis 1. Sabourauds dextrose agar sdahi media was prepared and sterilized for 15 minutes in autoclave at 121c. In addition, fungi make up part of the composite organisms called lichens.
Antifungal activity of some medicinal plants 53 agents were dissolved in dmso, 100% biologically inert substances. Antifungal activity of some medicinal plants used in. Moreover, the vast majority of studies were concerned only with the keratinophilic fungi which are important for. Incidence of keratinophilic fungi from selected soils of kerala state india. The present work reports the study of the distribution of keratinophilic fungi isolated from 39 samples of soil collected in brittany, france. The data show that only 39 of the 78 samples yielded keratinophilic fungi. A total of 19 fungi represented by 11 genera were isolated by the hairbaiting technique from 230 and 235 samples from hospitals and houses respectively. The present study is the first of its kind from jamaica. Keratinophilic fungi, dermatophytes, pathogenic fungi, keratin degradation, intensity of keratinolytic activity ika, polluted habitats summary key words keratinophilic fungi are important ecologically and interest in them is on the increase throughout the world 1,2. Pdf keratinophilic fungi inhabiting floor dusts of student houses at. Isolation and identification of microbial and fungal flora from female hair samples in riyadh saudi arabia. This species reported from students toe nails from egypt abdelhafez and elsharouny 1989.
Since then studies on the isolation of keratinophilic fungi from soil have been investigated throughout the world 4 7. Isolation of dermatophytes and other keratinophilic fungi. These organisms are surrounded by a rigid cell wall which is made up of chitin and can reproduce by sexual and asexual means alexopoulos et al. Relevant results are divided among a great many papers, often dealing primarily with other topics. These fungi are able to utilize keratin, a fibrous protein, as sole carbon and nitrogen source and survive saprophytically in nature english, 1963.
Study on keratinophilic and keratinolytic fungi isolated from. Pdf keratinophilic fungi are an ecologically important group of fungi that cycle one of the most abundant and highly stable animal proteins on earth. Keratinophilic fungi and related dermatophytes in polluted. The prevalence of keratinophilic and saprobic fungi on. Some of them share certain morphological features, constituting aspecial group called dermatophytes gugnani, 2000. Incidence of keratinophilic fungi from the selected soils. A survey of keratinophilic fungi as a tool for hen feather. During a collecting trip exploring new habitats and locations for keratinophilic fungi, soil samples were collected from bohani village in the.
Isolation and molecular identification of keratinophilic. Pdf a study on role of keratinophilic fungi in nature. The fungi are present almost in every ecological niche. The keratinophilic fungi are of prime importance in regard to various skin diseases prevalent in various areas.
These keratinophilic fungi produce extracellular enzymes keratinases which are capable of hydrolyzing keratin. Keratinophilic fungi were isolated from 65 of 110 samples 38 of marine soil and 72 of feathers and included chrysosporium tropicum from 36 samples, microsporum gypseum 17, c. A dilution of 103 was chosen for the estimation of the fungal total count and he hair bait t technique of vanbreuseghem 1952 was used to isolate keratinophilic fungi. Some are keratinolytic and play an important ecological role in decomposing.
The keratinophilic fungi leads to remarkably effective methods of control. These fungi therefore play a significant role in the breakdown of soil debris. They play a significant role in the natural degrada. While for the isolation of fungi from soil sediment two methods were used. Occurrence of keratinophilic fungi from soils of ujjain holy. These fungi are present in the environment with variable distribution patterns which depend on factors, such as human and or animal presence. Keratinophilic definition of keratinophilic by medical.
Approximately 500 isolations of nonkeratinophilic fungi were made. Pathogenic dermatophytes probably arose from soil borne nonpathogenic ancestors, possibly similar in habitat and adaptation to the human host, and. Their role in nature and degradation of keratinic substrates. Our study results showed that many keratinophilic fungi isolated from the parks soil are important for public health and children are an important group at a high risk of being exposed to these fungi. Dermatophytes, related keratinophilic and opportunistic fungi. Dermatophytes, related keratinophilic and opportunistic. Kingdom fungi the true fungi is a monophyletic group of eukaryotic heterotrophs that reproduce with spores and have chitinous cell walls. Study on keratinophilic and keratinolytic fungi isolated.
Their role in nature and degradation of keratinic substrates valeria filipello marchisio dipartimento di biologia vegetale, universita di torino, torino, italy keratinophilic fungi are natural colonizers of keratinic substrates. Several investigations on the dermatophytes and keratinophilic fungi on the hair of domestic. These fungi grow keratin substrates and degrade them. Several scattered reports of occurrence of these fungi in india are appearing. Keratinophilic fungi of the galapagos islands keratinophilic fungi of the galapagos islands ajello, l padhye, a. The lichens are actually mutualistic, symbiotic relationships between fungi and photosynthetic algae or photosynthetic cyanobacteria. A total of eleven species from seven genera were isolated. After that deshmukh and agrawal1983, pandey 1986 and rathore 1987 have done a good work on keratinophilic fungi. Keratinophilic fungi are natural colonizers of keratinic substrates. Biology of dermatophytes and other keratinophilic fungi. The prevalence of keratinophilic and saprobic fungi trichophyton terrestre was the only dermatophyte species found on the feathers of turkeys and was recovered from one sample out of 20 and had the riv of 5.
The hair samples were surface sterilized using 90% alcohol, for 2 minutes. Species diversity of keratinophilic fungi in various soil. Introduction keratinophilic fungi are ecologically an important group of. Study on keratinophilic and keratinolytic fungi isolated from birds feathers and animal hairs k. This chapter discusses the occurrence of keratinophilic fungi in different habitats deshmukh and verekar b, kerala deshmukh b, the andamans. A fungal penetrating organ was observed in situ, protruding from the shaft of the hair. The prevalence of keratinophilic fungi and aerobic actinomycetes in soils of gorgan, northern of iran has not been studied previously. Keratinophilic fungi on sheep hairs from the west bank of jordan. A total of nine species in four genera were isolated. Keratinophilic fungi are small, well defined and important group of fungi that colonize various keratinous substrates and degrade them to components of low molecular weight. The physiology of keratinophilic fungi has received relatively little attention, particularly over the last two decades. Fungi are eukaryotic, heterotrophic, nonphotosynthetic organisms in a separate kingdom of the same name. Arab river and its creeks and analysed for dermatophytes and related keratinophilic fungi.
Chrysosporium keratinophilum is a mold that is closely related to the dermatophytic fungi family arthrodermataceae and is mainly found in soil and the coats of wild animals to break down keratin. Keratinophilic fungi of the galapagos islands, mycoses. Keratinophilic fungi are an important group of fungi that live in soil. Diversity of keratinophilic fungi on human hairs and nails. Occurrence of keratinophilic fungi from soils of ujjain. Antifungal activity of some medicinal plants used in jeddah. These fungi are prevalent in soil, if it is in reached by human and animal derbies. The results revealed that out of 25 samples only 52% yielded dermatophytes and related keratinophilic fungi. One hundred keratinophilic fungi belonging to acremonium, aphanoascus, amauroascus, botryotrichum, chrysosporiurn, ctenomyces, gymnoascus, malbranchea.
Keratinophilic fungi grow on keratinous substances which is important natural material occurring in nature mainly in the form of hairs, wools, feathers, horns, hooves, nails, skin and other cornified appendages, constituting natural baits for these fungi khanam and jain. A total of 19 fungi represented by 11 genera were isolated by the hairbaiting technique from 230. The impact of keratinophilic fungi on human health seems unexplored part of various studies shukia et al. The aim of this study was to investigate the influence of culture conditions on growth of keratinophilic fungal strain of chrysosporium sp. This report represents the first study of keratinophilic fungi present in soils of jamaica. On hair digestion is carried out primarily by means of flat fronds of eroding mycelium which first life the cuticular scales and then erode the cortex.
Chrysosporium keratinophilum is one of the more commonly occurring species of the genus chrysosporium in nature. The most familiar fungi are kitchen molds and mushrooms. Structurally, fungi are eukaryotes which may be unicellular or multicellular. Indian keratinophilic fungal flora was described by tripathi and kushwaha1 who describe 270 fungal species.
The present study was performed to determine the prevalence of keratinophilic fungi and bacteria from hair samples of femalesfrom november 2016 to april 2017. Dermatophytes, related keratinophilic and opportunistic fungi were isolated from indoor dust samples of 46 hospitals and 47 houses in kanpur. Keratinophilic fungi from marine soils of bombay, india. Keratinophilic fungi from soil of brittany, france. Determination of minimal inhibitory concentration of plant extract on fungal growth the mic was determined by the methods described by chand et al. Keratinophilic fungi distribution, 6 pathogenicity and biotechnological potentials shilpa a. Isolation of keratinophilic fungi and aerobic actinomycetes.